{UTF-8}
"V(max)"=
	k2*Total Enzyme
	~	
	~		|

Total Enzyme=
	Enzyme+ES complex
	~	
	~		~	:SUPPLEMENTARY 
	|

Km=
	("k-1"+k2)/(k1+1e-016)
	~	
	~	+0.0000000000000001 is to prevent division by zero!!!!!!!
	|

Enzyme= INTEG (
	rate 1b ES+rate 2 ES-rate 1 E,
		0.2)
	~	
	~		|

ES complex= INTEG (
	rate 1 E-rate 1b ES-rate 2 ES,
		0)
	~	
	~		|

ES complex 2= INTEG (
	rate 1 S-rate 1b ES2-rate 2 ES2,
		0)
	~	
	~		|

"k-1"=
	0.01
	~	 [0,0.5,0.01]
	~		|

k1=
	0.01
	~	 [0,1,0.01]
	~		|

k2=
	0.01
	~	 [0,1,0.01]
	~		|

Product= INTEG (
	rate 2 ES2,
		0)
	~	
	~		|

rate 1 E=
	k1*Enzyme*Substrate
	~	
	~		|

rate 1 S=
	k1*Enzyme*Substrate
	~	
	~		|

rate 1b ES=
	"k-1"*ES complex
	~	
	~		|

rate 1b ES2=
	"k-1"*ES complex 2
	~	
	~		|

rate 2 ES=
	k2*ES complex
	~	
	~		|

rate 2 ES2=
	k2*ES complex 2
	~	
	~		|

Substrate= INTEG (
	rate 1b ES2-rate 1 S,
		1)
	~	
	~		|

********************************************************
	.Control
********************************************************~
		Simulation Control Parameters
	|

FINAL TIME  = 30
	~	Second
	~	The final time for the simulation.
	|

INITIAL TIME  = 0
	~	Second
	~	The initial time for the simulation.
	|

SAVEPER  = 
        TIME STEP
	~	Second [0,?]
	~	The frequency with which output is stored.
	|

TIME STEP  = 0.125
	~	Second [0,?]
	~	The time step for the simulation.
	|

\\\---/// Sketch information - do not modify anything except names
V300  Do not put anything below this section - it will be ignored
*View 1
$192-192-192,0,Times New Roman|12||0-0-0|0-0-0|0-0-255|-1--1--1|-1--1--1|96,96,100,0
10,1,Enzyme,204,215,40,20,3,3,0,0,0,0,0,0
10,2,ES complex,480,386,40,20,3,3,0,0,0,0,0,0
10,3,ES complex 2,480,515,40,20,3,3,0,0,0,0,0,0
10,4,Substrate,199,628,40,20,3,3,0,0,0,0,0,0
10,5,Product,750,515,40,20,3,3,0,0,0,0,0,0
1,6,8,2,4,0,0,22,0,0,0,-1--1--1,,1|(476,294)|
1,7,8,1,100,0,0,22,0,0,0,-1--1--1,,1|(357,215)|
11,8,460,476,215,6,8,34,3,0,0,3,0,0,0
10,9,rate 1 E,476,196,28,11,40,3,0,0,-1,0,0,0
1,10,12,1,4,0,0,22,0,0,0,-1--1--1,,1|(204,306)|
1,11,12,2,100,0,0,22,0,0,0,-1--1--1,,1|(325,386)|
11,12,380,204,386,6,8,34,3,0,0,1,0,0,0
10,13,rate 1b ES,204,405,35,11,40,3,0,0,-1,0,0,0
1,14,16,1,4,0,0,22,0,0,0,-1--1--1,,1|(205,147)|
1,15,16,2,100,0,0,22,0,0,0,-1--1--1,,4|(732,92)|(732,92)|(732,386)|(626,386)|
11,16,348,205,92,6,8,34,3,0,0,3,0,0,0
10,17,rate 2 ES,205,73,35,11,40,3,0,0,-1,0,0,0
1,18,20,3,4,0,0,22,0,0,0,-1--1--1,,1|(322,515)|
1,19,20,4,100,0,0,22,0,0,0,-1--1--1,,1|(199,565)|
11,20,364,199,515,6,8,34,3,0,0,3,0,0,0
10,21,rate 1 S,199,496,28,11,40,3,0,0,-1,0,0,0
1,22,24,4,4,0,0,22,0,0,0,-1--1--1,,1|(356,627)|
1,23,24,3,100,0,0,22,0,0,0,-1--1--1,,1|(480,577)|
11,24,412,480,627,6,8,34,3,0,0,1,0,0,0
10,25,rate 1b ES2,480,646,41,11,40,3,0,0,-1,0,0,0
1,26,28,5,4,0,0,22,0,0,0,-1--1--1,,1|(665,515)|
1,27,28,3,100,0,0,22,0,0,0,-1--1--1,,1|(564,515)|
11,28,428,615,515,6,8,34,3,0,0,1,0,0,0
10,29,rate 2 ES2,615,534,39,11,40,3,0,0,-1,0,0,0
10,30,k1,52,348,20,20,2,3,0,0,1,0,0,0
10,31,"k-1",52,443,20,20,2,3,0,0,1,0,0,0
10,32,k2,857,424,20,20,2,3,0,0,1,0,0,0
1,33,2,16,1,0,0,0,0,64,0,-1--1--1,,1|(538,170)|
1,34,32,16,1,0,0,0,0,64,0,-1--1--1,,1|(677,147)|
1,35,3,29,1,0,0,0,0,64,0,-1--1--1,,1|(538,563)|
1,36,32,29,1,0,0,0,0,64,0,-1--1--1,,1|(790,570)|
1,37,3,25,1,0,0,0,0,64,0,-1--1--1,,1|(387,580)|
1,38,4,20,1,0,0,0,0,64,0,-1--1--1,,1|(135,547)|
1,39,1,20,1,0,0,0,0,64,0,-1--1--1,,1|(111,408)|
1,40,1,8,1,0,0,0,0,64,0,-1--1--1,,1|(361,165)|
1,41,4,8,1,0,0,0,0,64,0,-1--1--1,,1|(8,310)|
1,42,30,8,0,0,0,0,0,64,0,-1--1--1,,1|(263,281)|
1,43,30,20,1,0,0,0,0,64,0,-1--1--1,,1|(98,451)|
1,44,31,24,1,0,0,0,0,64,0,-1--1--1,,1|(143,712)|
1,45,31,13,0,0,0,0,0,64,0,-1--1--1,,1|(114,427)|
1,46,2,13,1,0,0,0,0,64,0,-1--1--1,,1|(338,438)|
12,47,157484408,811,760,259,178,3,188,0,0,1,0,0,0
conc.
10,48,Total Enzyme,620,450,20,20,2,3,0,0,1,0,0,0
1,49,1,48,0,0,0,0,0,64,0,-1--1--1,,1|(414,334)|
1,50,2,48,0,0,0,0,0,64,0,-1--1--1,,1|(554,419)|
10,51,Km,830,123,20,20,2,3,0,0,1,0,0,0
1,52,30,51,0,0,0,0,0,64,0,-1--1--1,,1|(433,237)|
1,53,31,51,0,0,0,0,0,64,0,-1--1--1,,1|(434,285)|
1,54,32,51,0,0,0,0,0,64,0,-1--1--1,,1|(844,280)|
10,55,"V(max)",740,435,20,20,2,3,0,0,1,0,0,0
1,56,48,55,0,0,0,0,0,64,0,-1--1--1,,1|(672,443)|
1,57,32,55,0,0,0,0,0,64,0,-1--1--1,,1|(805,428)|
\\\---/// Sketch information - do not modify anything except names
V300  Do not put anything below this section - it will be ignored
*View 2
$192-192-192,0,Comic Sans MS|12||0-0-255|0-0-0|0-0-255|-1--1--1|-1--1--1|96,96,5,0
12,1,470156726,778,518,437,313,3,188,0,0,1,0,0,0
conc.
12,2,16778884,448,879,109,37,3,252,0,0,0,0,0,0
k1,0,1,0.01
12,3,158467448,668,878,108,38,3,252,0,0,0,0,0,0
"k-1",0,0.5,0.01
12,4,12126108,890,879,105,38,3,252,0,0,0,0,0,0
k2,0,1,0.01
12,5,114952112,1110,877,109,38,3,252,0,0,0,0,0,0
TIME STEP,0,1,0.01
12,6,0,535,182,59,13,8,132,0,46,-1,0,0,0,0-0-0,0-0-255,Comic Sans MS|14||255-255-255
 substrate, S 
12,7,0,679,182,49,13,8,132,0,46,-1,0,0,0,0-0-0,255-0-0,Comic Sans MS|14||255-255-255
 enzyme, E 
12,8,0,852,183,72,13,8,132,0,46,-1,0,0,0,0-0-0,0-128-0,Comic Sans MS|14||255-255-255
 ES complex, ES 
12,9,0,1012,182,49,13,8,132,0,46,-1,0,0,0,0-0-0,128-128-128,Comic Sans MS|14||255-255-255
 product, P 
12,10,0,763,30,189,36,8,132,0,58,-1,0,0,0,0-0-0,0-0-0,Comic Sans MS|30|B|0-128-0
Enzyme Kinetics
12,11,0,760,112,297,40,8,132,0,42,-1,0,0,0,0-0-0,0-0-0,Comic Sans MS|24||128-64-0
S   +    E   < --->    ES    --->   P   +   E
12,12,0,682,97,10,11,8,4,0,0,-1,0,0,0
k1
12,13,0,688,134,13,11,8,4,0,0,-1,0,0,0
k-1
12,14,0,854,93,13,8,8,132,0,0,-1,0,0,0
k2
12,15,0,276,432,50,15,8,132,0,8,2,0,0,0,0-0-0,0-0-0,|18||0-0-0
(S)o  =  1 
12,16,0,186,502,75,16,8,4,0,10,-1,0,0,0,0-0-0,0-0-0,|18||255-0-0
 (E)o   =   0.2
///---\\\
:GRAPH conc.
:TITLE Concentrations for Enzyme Reaction
:X-AXIS Time
:SCALE
:VAR Substrate|Substrate
:Y-MIN 0
:Y-MAX 1
:LINE-WIDTH 3
:VAR Enzyme|Enzyme
:Y-MIN 0
:Y-MAX 1
:LINE-WIDTH 3
:VAR ES complex|ES complex
:Y-MIN 0
:Y-MAX 1
:LINE-WIDTH 3
:VAR Product|Product
:Y-MIN 0
:Y-MAX 1
:LINE-WIDTH 3
:VAR Total Enzyme|Total enzyme
:Y-MIN 0
:Y-MAX 1
:LINE-WIDTH 1

:TABLE table
:TITLE table
:PRETTYNUM
:TIME-DOWN
:FIRST-CELLWIDTH 30
:CELLWIDTH 14
:FONT Times New Roman|12||0-0-0
:VAR ES complex
:VAR ES complex 2

:TABLE GRAPH
:TITLE GRAPH
:PRETTYNUM
:TIME-DOWN
:FIRST-CELLWIDTH 30
:CELLWIDTH 14
:FONT Times New Roman|12||0-0-0
:VAR Km
:VAR "V(max)"
:L<%^E!@
9:Current
23:0
15:0,0,0,1,0,0
19:5,1
27:2,
34:0,
4:Time
5:ES complex
24:0
25:30
26:30