{UTF-8}
Enzyme= INTEG (
	enzyme release-forward rate+enzyme release 2,
		0.2)
	~	
	~		|

enzyme release=
	reverse rate constant*ES Complex
	~	
	~		|

enzyme release 2=
	forward 2 rate constant*ES Complex
	~	
	~		|

ES Complex= INTEG (
	forward rate-0.5 * (product formation+enzyme release 2)-0.5 * (enzyme release+substrate release\
		),
		0)
	~	
	~		|

forward rate=
	forward rate constant*Enzyme*Substrate
	~	
	~		|

Substrate= INTEG (
	substrate release-forward rate,
		1)
	~	
	~		|

product formation=
	forward 2 rate constant*ES Complex
	~	
	~		|

forward 2 rate constant=
	1
	~	 [0,2,0.1]
	~		|

forward rate constant=
	1
	~	 [0,2,0.1]
	~		|

Product= INTEG (
	product formation,
		0)
	~	
	~		|

substrate release=
	reverse rate constant*ES Complex
	~	
	~		|

reverse rate constant=
	0.3
	~	 [0,0.5,0.05]
	~		|

********************************************************
	.Control
********************************************************~
		Simulation Control Parameters
	|

FINAL TIME  = 30
	~	Second
	~	The final time for the simulation.
	|

INITIAL TIME  = 0
	~	Second
	~	The initial time for the simulation.
	|

SAVEPER  = 
        TIME STEP
	~	Second [0,?]
	~	The frequency with which output is stored.
	|

TIME STEP  = 0.125
	~	Second [0,?]
	~	The time step for the simulation.
	|

\\\---/// Sketch information - do not modify anything except names
V300  Do not put anything below this section - it will be ignored
*View 1
$192-192-192,0,Times New Roman|12||0-0-0|0-0-0|0-0-255|-1--1--1|-1--1--1|120,120,5,0
10,1,ES Complex,667,392,56,29,3,131,0,0,0,0,0,0
10,2,Product,1107,394,50,25,3,3,0,0,0,0,0,0
10,3,Enzyme,377,244,50,25,3,3,0,0,0,0,0,0
10,4,Substrate,369,585,50,25,3,3,0,0,0,0,0,0
1,5,7,2,4,0,0,22,0,0,0,-1--1--1,,1|(978,394)|
1,6,7,1,100,0,0,22,0,0,0,-1--1--1,,1|(803,394)|
11,7,300,892,394,8,10,34,3,0,0,1,0,0,0
10,8,product formation,892,437,83,33,40,131,0,0,-1,0,0,0
1,9,11,3,4,0,0,22,0,0,0,-1--1--1,,1|(525,254)|
1,10,11,1,100,0,0,22,0,0,0,-1--1--1,,1|(631,313)|
11,11,252,631,254,8,10,34,3,0,0,1,0,0,0
10,12,enzyme release,631,277,60,14,40,3,0,0,-1,0,0,0
1,13,15,4,4,0,0,22,0,0,0,-1--1--1,,1|(541,580)|
1,14,15,1,100,0,0,22,0,0,0,-1--1--1,,1|(672,495)|
11,15,156,672,580,8,10,34,3,0,0,1,0,0,0
10,16,substrate release,672,622,71,32,40,131,0,0,-1,0,0,0
10,17,forward rate constant,147,394,50,24,8,3,0,0,0,0,0,0
10,18,reverse rate constant,842,644,48,24,8,3,0,0,0,0,0,0
10,19,forward 2 rate constant,1020,186,58,24,8,3,0,0,0,0,0,0
1,20,18,15,1,0,0,0,0,64,0,-1--1--1,,1|(762,577)|
1,21,1,15,1,0,0,0,0,64,0,-1--1--1,,1|(727,495)|
1,22,24,1,4,0,0,22,0,0,0,-1--1--1,,1|(491,387)|
1,23,24,3,100,0,0,22,0,0,0,-1--1--1,,1|(364,323)|
11,24,236,364,387,8,10,34,3,0,0,1,0,0,0
10,25,forward rate,364,398,54,1,40,131,0,0,-1,0,0,0
10,26,reverse rate constant,589,106,54,24,8,2,0,3,-1,0,0,0,128-128-128,0-0-0,|12||128-128-128
1,27,1,12,1,0,0,0,0,64,0,-1--1--1,,1|(607,302)|
1,28,1,7,1,0,0,0,0,64,0,-1--1--1,,1|(791,350)|
1,29,19,7,1,0,0,0,0,64,0,-1--1--1,,1|(1025,325)|
12,30,0,972,874,359,189,3,188,0,0,1,0,0,0
Amounts
12,31,0,1764,522,311,244,3,188,0,0,1,0,0,0
Table
12,32,0,309,874,296,189,3,188,0,0,1,0,0,0
Product
1,33,24,4,100,0,0,22,0,0,0,-1--1--1,,1|(364,478)|
1,34,4,24,1,0,0,0,0,64,0,-1--1--1,,1|(447,528)|
1,35,3,25,1,0,0,0,0,64,0,-1--1--1,,1|(281,338)|
1,36,17,25,0,0,0,0,0,64,0,-1--1--1,,1|(246,395)|
1,37,26,12,1,0,0,0,0,64,0,-1--1--1,,1|(567,231)|
1,38,40,3,4,0,0,22,0,0,0,-1--1--1,,1|(377,168)|
1,39,40,1,100,0,0,22,0,0,0,-1--1--1,,1|(755,392)|
11,40,0,755,168,8,10,34,3,0,0,1,0,0,0
10,41,enzyme release 2,755,192,68,14,40,3,0,0,0,0,0,0
1,42,1,40,0,0,0,0,0,64,0,-1--1--1,,1|(712,275)|
1,43,19,40,0,0,0,0,0,64,0,-1--1--1,,1|(868,176)|
///---\\\
:GRAPH Amounts
:TITLE Amounts Enzyme
:X-AXIS Time
:SCALE
:VAR Enzyme
:Y-MIN 0
:Y-MAX 0.25
:LINE-WIDTH 3
:VAR ES Complex
:LINE-WIDTH 3

:TABLE Table
:TITLE Table
:PRETTYNUM
:TIME-DOWN
:FIRST-CELLWIDTH 18
:CELLWIDTH 18
:FONT Times New Roman|9||0-0-0
:VAR Enzyme
:VAR Substrate
:VAR Product
:VAR ES Complex

:GRAPH Product
:TITLE Product
:X-AXIS Time
:SCALE
:VAR Substrate
:LINE-WIDTH 3
:VAR Product
:LINE-WIDTH 3
:L<%^E!@
1:Current.vdf
9:Current
23:0
15:0,0,0,0,0,0
19:5,0
27:2,
34:0,
4:Time
5:substrate release
24:0
25:30
26:30