Angelique Bosse
Montgomery Blair High School
Silver Spring, Maryland 20901
Gene sequences can be accessed in either of the following two approaches:
-
Go to Entrez at
http://www.ncbi.nlm.nih.gov/Entrez/
- Click on "Nucleotide" (part of the diagram)
- In the skinny box next to "Search for" type in a protein name such as
"phenylalanine
hydroxylase"
- Click on "Go"
- The result is a list of various types of the requested sequences
- Click on any of the accession numbers displayed and you will be able
to
view information about
that sequence such as definition, source, classification, journal
articles, comments, amino
acid sequence, and the nucleotide sequence.
OR
-
Go to Biology Workbench at
http://workbench.sdsc.edu/
- Click "Enter the Biology Workbench 3.2" and enter user name and
password, click "ok"
(or set up a free account first)
- Scroll down and click "Nucleic Tools"
- Select "Ndjinn- Multiple Database Search"
- Click "Run"
- In the blank box next to "Exact Match" type in a protein such as
"phenylalanine hydroxylase"
- Select "Show 10 Hits"
- Scroll down to select a database, click in the little white box next
to
your choice, such as
"GBPRI- GenBank Primate Sequences"
- Click "Search"
- Result is a list of "hits" for your requested sequence under "Matching
Database Record"
- Select a record by clicking the little white box next to all EXCEPT
the
one you want (a check
mark should only be next to your choice)
- Select "Show Record(s)"
- Result is information about the selected sequence such as locus,
definition, source, references,
base count, and amino acid sequence.
- To view the nucleic acid sequence, scroll to the bottom of the screen
and
click "Show
Sequence(s)"
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