Gene Sequence Search  

Angelique Bosse
Montgomery Blair High School
Silver Spring, Maryland 20901

Gene sequences can be accessed in either of the following two approaches:

  • Go to Entrez at http://www.ncbi.nlm.nih.gov/Entrez/
  • Click on "Nucleotide" (part of the diagram)
  • In the skinny box next to "Search for" type in a protein name such as "phenylalanine hydroxylase"
  • Click on "Go"
  • The result is a list of various types of the requested sequences
  • Click on any of the accession numbers displayed and you will be able to view information about that sequence such as definition, source, classification, journal articles, comments, amino acid sequence, and the nucleotide sequence.
OR
  • Go to Biology Workbench at http://workbench.sdsc.edu/
  • Click "Enter the Biology Workbench 3.2" and enter user name and password, click "ok" (or set up a free account first)
  • Scroll down and click "Nucleic Tools"
  • Select "Ndjinn- Multiple Database Search"
  • Click "Run"
  • In the blank box next to "Exact Match" type in a protein such as "phenylalanine hydroxylase"
  • Select "Show 10 Hits"
  • Scroll down to select a database, click in the little white box next to your choice, such as "GBPRI- GenBank Primate Sequences"
  • Click "Search"
  • Result is a list of "hits" for your requested sequence under "Matching Database Record"
  • Select a record by clicking the little white box next to all EXCEPT the one you want (a check mark should only be next to your choice)
  • Select "Show Record(s)"
  • Result is information about the selected sequence such as locus, definition, source, references, base count, and amino acid sequence.
  • To view the nucleic acid sequence, scroll to the bottom of the screen and click "Show Sequence(s)"

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