Angelique Bosse
Montgomery Blair High School
Silver Spring, Maryland 20901
-
Go to Entrez at
http://www.ncbi.nlm.nih.gov/Entrez/
- Click on "Nucleotide" (part of the flow chart)
- In the skinny box next to "Search for" type in a protein name such as
"phenylalanine
hydroxylase"
- Click on "Go"
- The result is a list of various types of the protein
- Click on any of the accession numbers displayed and you will be able
to
view information about
that sequence such as definition, source, classification, journal
articles, comments, amino
acid sequence, and the nucleotide sequence.
- Scroll down to the bottom of the page to see the nucleotide sequence.
- "Copy" the sequence (you will be pasting it at another site)
- Go to
http://tools.neb.com/NEBcutter2/index.php
- Paste in your DNA sequence.
- Click on Submit.
- Result is a description of your sequence including size, number of
each
type of base. Also, a list
of restriction endonucleases which cut your sequence, a list by base # of
enzymes that
cut and how many times they cut, and restriction cuts sorted by enzyme in
alphabetical
order.
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