Angelique Bosse
Montgomery Blair High School
Silver Spring, Maryland 20901
-
Go to Biology Workbench at http://workbench.sdsc.edu/
- Click "Enter the Biology Workbench 3.2" and enter user name and
password, click "ok"
(or set up a free account first)
- Scroll down and click "Protein Tools"
- Select "Ndjinn- Multiple Database Search"
- Click "Run"
- In the skinny white box next to "Exact Match" type in a protein such
as
"beta hemoglobin" and
select "Show 10 Hits"
- Scroll down to choose a database such as "SWISSPROT- Swissprot
Database"
by clicking in
white box
- Go back up to top of screen and click "Search"
- Result is a list of "hits" for your requested protein under "Matching
Database Record".
- When the list of "hits" is produced, select one to import. Clear all
the
little white boxes next to
the hits EXCEPT for your selection. (Only the white box next to your
selection should
have a check mark).
- Click "Import Sequence(s)"
- From the menu list, scroll down and click "GOR4- Predict Secondary
Structure of PS"
- Click the white box next to your imported sequence
- Click "Run"
- On next screen click "Submit"
- Scroll down to see color-coded amino acid sequence. Refer to the key
below the amino acid
sequence which indicates that red is alpha helix, blue is beta sheet, and
black is random coil
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