Secondary Structure of a Protein 

Angelique Bosse
Montgomery Blair High School
Silver Spring, Maryland 20901

  • Go to Biology Workbench at
  • Click "Enter the Biology Workbench 3.2" and enter user name and password, click "ok" (or set up a free account first)
  • Scroll down and click "Protein Tools"
  • Select "Ndjinn- Multiple Database Search"
  • Click "Run"
  • In the skinny white box next to "Exact Match" type in a protein such as "beta hemoglobin" and select "Show 10 Hits"
  • Scroll down to choose a database such as "SWISSPROT- Swissprot Database" by clicking in white box
  • Go back up to top of screen and click "Search"
  • Result is a list of "hits" for your requested protein under "Matching Database Record".
  • When the list of "hits" is produced, select one to import. Clear all the little white boxes next to the hits EXCEPT for your selection. (Only the white box next to your selection should have a check mark).
  • Click "Import Sequence(s)"
  • From the menu list, scroll down and click "GOR4- Predict Secondary Structure of PS"
  • Click the white box next to your imported sequence
  • Click "Run"
  • On next screen click "Submit"
  • Scroll down to see color-coded amino acid sequence. Refer to the key below the amino acid sequence which indicates that red is alpha helix, blue is beta sheet, and black is random coil

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